Downstream Processing Lab Practical

Downstream processing (also known as DSP) refers to the recovery and purification of a drug substance (DS) from natural sources, such as animal or bacterial cells. It is defined as the unit operations that follow cell growth and expansion; with a purpose to isolate, purify, and concentrate the previously synthesized drug substance or other product from the complex bulk matrix.  In this course, students will learn separation techniques with hands-on experience using a small-scale Akta Prime chromatography system to perform both Anion Exchange and Hydrophobic Interaction Chromatography.

Audience
  • Bioprocess Technicians
  • Bioprocess Engineers
  • Manufacturing Associates
  • Purification Technicians
  • Purification Lab Analysts
  • Chromatography Associates
Topics

Day 1

  1. Introduction
  2. Lab: AktaPrime Chromatography ID
  3. Process Operation of GFP
  4. GFP Chromatography Run

Day 2

  1. Introduction
  2. Lab: Process Operation of GFP
  3. GFP Chromatography Run
Objectives
  1. Learn about Anion Exchange chromatography.
  2. Learn about Hydrophobic Interaction   chromatography.
  3. Perform hands-on techniques in small scale chromatography.
Files
Detailed Course Outline
Chromatography in Theory & Practice
College Options
Wake Technical Community College
Delivery Options
Face-to-Face

Delivered in-person in a classroom or lab setting.

Contact Hours
Level

Intermediate

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