High Performance Liquid Chromotography (HPLC)

High Performance Liquid Chromotography (HPLC)


Explore the concepts of High Performance Liquid Chromatography (HPLC) by analyzing and comparing beverages to determine which has more caffeine. In this activity, you will learn more about how HPLC works by testing each sample and examining the chromatograms to find the beverage with the highest caffeine content.

Learning Objectives

Upon completion of this exercise, you will be able to:

  1. Discuss the difference between mobile phase and stationary phase 
  2. Identify key components of the HPLC system
  3. Make observations by reading the chromatograms
  4. Choose the beverage with the most caffeine using your observations
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