This course provides students with a sound, practical, and theoretical knowledge of key techniques to perform cell culture. Topics include growth environment, routine maintenance of cell cultures, cryopreservation, transfection, and cell counting. Other topics include cloning, techniques, suspension culture, serum-free culture, evaluation of cryopreserved cells, extraction of DNA from mammalian cells, and use of multi-well plates in cell culture. Upon completion, students should be able to demonstrate the knowledge and skills required to initiate, maintain, and manipulate cells in culture. Co-op opportunity available.