Quantitative Spectrophotometry

Quantitative Spectrophotometry
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Target Audience: High School
NCCCS Core Courses: CHM-115
NC DPI Standards: Chm.3.2, Chm.3.2.1, Chm.3.2.2, Chm.3.2.3, Chm.3.2.4, Chm.3.2.5, Chm.3.2.6
Equipment Used: Vernier LabQuest Unit, UV Visible
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Spectrophotometers measure the effect of a sample on a beam of light. This information can provide scientists with quantitative data regarding the amount of analyte present in a sample such as the amount of FD&C dyes found in popular foods and drinks. In this activity, students will create a standard curve that they will then use to measure the analyte concentration of unknown samples.

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