Downstream

Downstream processing in biomanufacturing is a multi-step process of recovery and purification.

Downstream

Downstream processing in biomanufacturing is a multi-step process of recovery and purification of desired products from a complex mixture of cell proteins, nucleic acids, membranous material, sugars and salts. A number of important pharmaceutical products such as insulin, antibiotics, monoclonal antibodies (Mab’s) and vaccines are purified through downstream processing methodologies. The required purity and efficacy of drug products can be achieved by various separation and purification steps such as centrifugation, diafiltration, ultrafiltration and chromatography; a combination of two or three of these processes, based on the physical and chemical properties of the desired product, are utilized for successful isolation and purification of the product. Students will learn fundamental recovery and purification concepts via hands-on training in sample preparation, high performance liquid chromatography, column packing, column performance testing, instrument calibration, ion exchange chromatography and hydrophobic interaction chromatography. 

Online Courses

BioNetwork has several online courses.

Instrumentation Calibration Program for the Life Science Industry
This course reviews the regulatory requirements and methods for determining calibration... more
Advanced Batch Production Records Concepts
Participants will learn the importance and benefits of having and completing Batch Production... more
Micropipetting Techniques for Precision & Accuracy
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Working With Proteins
Video
Denaturation is a process that causes a protein to unfold and lose its shape, and it usually... more
BioWork 5060: Plate & Frame Filtration
Video
Plate and frame filtration is widely used in food, beverage, pharma and chemical manufacturing. Let... more
BioWork 5070: Column Chromatography Separation (Grape Soda)
Video
Chromatography is a common technique to purify a pharma product. It’s how we break down a complex... more
7040 Sterilizing Liquids - Autoclaving and Filtration
Video
The autoclave and sterile filtration are two common techniques we use to sterilize liquids and... more