Downstream

Downstream processing in biomanufacturing is a multi-step process of recovery and purification.

Downstream

Downstream processing in biomanufacturing is a multi-step process of recovery and purification of desired products from a complex mixture of cell proteins, nucleic acids, membranous material, sugars and salts. A number of important pharmaceutical products such as insulin, antibiotics, monoclonal antibodies (Mab’s) and vaccines are purified through downstream processing methodologies. The required purity and efficacy of drug products can be achieved by various separation and purification steps such as centrifugation, diafiltration, ultrafiltration and chromatography; a combination of two or three of these processes, based on the physical and chemical properties of the desired product, are utilized for successful isolation and purification of the product. Students will learn fundamental recovery and purification concepts via hands-on training in sample preparation, high performance liquid chromatography, column packing, column performance testing, instrument calibration, ion exchange chromatography and hydrophobic interaction chromatography. 

Online Courses

BioNetwork has several online courses.

BioCertification Boot Camp
All students who complete the BioWork Certificate Program successfully are eligible to take the... more
hplc
High performance liquid chromatography (HPLC) is a chemistry technique used to separate compounds... more
Operations in Biotechnology Processes: An Overview

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Autoclave Operation
eLearning
In this interactive lesson, we'll cover an overview of the autoclave, review safety considerations... more
Batch Documents
eLearning
In this interactive exercise, you will learn the importance of batch documents by reviewing the... more
Bioprocessing Part 2: Separation / Recovery
Video
This video is the second in a series of three videos depicting the major stages of industrial-scale... more
Bioprocessing Part 3: Purification
Video
This video is the third in a series of three videos depicting the major stages of industrial-scale... more
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