Sample Preparation for HPLC Analysis

Sample Preparation for HPLC Analysis

Students will execute basic analytical processes and use HPLC (High Performance Liquid Chromatography) instrumentation to perform analysis of trace organic compounds. You will learn to analyze biological samples for trace levels of drug and pesticide residues using HPLC. Students will perform standard preparation, reagent preparation, and sample extractions for HPLC analysis. Participants also learn about calibrating an HPLC system as well learn to follow a method developed to determine trace levels of an organic contaminant in biological samples.

This course must be taught at a BioNetwork location.
Level: Intermediate
Lecture Hours: 8.00
Lab Hours: 8.00
Total Contact Hours: 16.00

Request this Course

Request this Course

Submit this form to request this course to be scheduled.

Image CAPTCHA
Enter the characters shown in the image.
Currently Offered
Sample Preparation for HPLC Analysis - Class begins May 2, 2019

Topics Include

  • Basics of High Performance Liquid Chromatography (HPLC)
  • Use of top loading and analytical balances, pipettes, and volumetric flasks
  • Introduction to bio-analytical (trace organic) analysis
  • Sampling, sample extraction, and separatory techniques, including solid phase extraction and solvent partitioning techniques
  • Standard solution preparations
  • HPLC auto-injection of calibration standards on an overnight run
  • Calibration of an HPLC unit
  • Importance of lab controls and lab-fortified controls as quality control checks
  • Demonstration of Method M 002 to determine a trace-level organic contaminant in biological samples
  • Challenges posed by real-world samples

Learning Objectives

  • Demonstrate competency in extracting trace samples from complex biological matrices.
  • Execute basic analytical processes and use HPLC instrumentation to perform trace organic analysis.

Audience

We designed this course for experienced professionals, including:

  • Laboratory Technician
  • Analytical Chemist
  • HPLC Scientist
  • Associate Scientist
  • Quality Control Analyst
  • Research and Development Personnel

Related Content

Current Courses
View All
March
21
HPLC in Theory & Practice
High performance liquid chromatography (HPLC) is a chemistry technique used to separate compounds of interest from a liquid mixture based on chemical and/or physical properties. When HPLC is coupled with a spectrophotometric detector, it can be used to quantify analytes to provide detailed
June
24
Biomanufacturing Bootcamp
This course allows community college instructors to experience industrial biotechnology through lectures and hands-on production of green fluorescent protein (GFP) within a current good manufacturing practices (cGMP) environment. The Capstone Center is a fully functional facility containing state-
Current Courses
View All
March
21
HPLC in Theory & Practice
High performance liquid chromatography (HPLC) is a chemistry technique used to separate compounds of interest from a liquid mixture based on chemical and/or physical properties. When HPLC is coupled with a spectrophotometric detector, it can be used to quantify analytes to provide detailed
June
24
Biomanufacturing Bootcamp
This course allows community college instructors to experience industrial biotechnology through lectures and hands-on production of green fluorescent protein (GFP) within a current good manufacturing practices (cGMP) environment. The Capstone Center is a fully functional facility containing state-
eLearning
View All
High Performance Liquid Chromotography (HPLC)
High Performance Liquid Chromotography (HPLC)
Learn the high level concepts of High Performance Liquid Chromatography by finding out which has more caffeine - an energy drink or coffee? Learn how HPLC works by testing each drink, and then examine the resulting to chromatograms to find out.
Virtual Microscope
Virtual Microscope
BioNetwork’s Virtual Microscope is the first fully interactive 3D scope - it’s a great practice tool to prepare you for working in a science lab. Learn terminology, usage, and care by interacting with a fully functional, virtual microscope. When you're ready, challenge your knowledge in the testing
Microscope Basics
Microscope Basics
Take a self-guided exploration of a common laboratory microscope including its care, components, and usage. 
  •  
  • 1 of 3
  • >
eLearning
View All
Virtual Microscope
Virtual Microscope
BioNetwork’s Virtual Microscope is the first fully interactive 3D scope - it’s a great practice tool to prepare you for working in a science lab. Learn terminology, usage, and care by interacting with a fully functional, virtual microscope. When you're ready, challenge your knowledge in the testing
Microscope Basics
Microscope Basics
Take a self-guided exploration of a common laboratory microscope including its care, components, and usage. 
Centrifugation
Centrifugation
This module includes, the basics of centrifugation, effects of gravity on particles in suspension, operation of a centrifuge, differential centrifugation, basic components, hazards and safety.
  •  
  • 1 of 3
  • >
eLearning
View All
Virtual Microscope
Virtual Microscope
BioNetwork’s Virtual Microscope is the first fully interactive 3D scope - it’s a great practice tool to prepare you for working in a science lab. Learn terminology, usage, and care by interacting with a fully functional, virtual microscope. When you're ready, challenge your knowledge in the testing
Centrifugation
Centrifugation
This module includes, the basics of centrifugation, effects of gravity on particles in suspension, operation of a centrifuge, differential centrifugation, basic components, hazards and safety.
High Performance Liquid Chromotography (HPLC)
High Performance Liquid Chromotography (HPLC)
Learn the high level concepts of High Performance Liquid Chromatography by finding out which has more caffeine - an energy drink or coffee? Learn how HPLC works by testing each drink, and then examine the resulting to chromatograms to find out.
  •  
  • 1 of 3
  • >
Videos
View All
How does a spectrophotometer work?
How does a spectrophotometer work?
This short animation demonstrates the inner workings of a spectrophotometer. Here's how a spectrophotometer works. A lamp provides the source of light. The beam of light strikes the diffraction grating, which works like a prism and separates the light into its component wavelengths. The grating is
Gel Electrophoresis
Gel Electrophoresis
This animation explores the fundamentals of gel electrophoresis by covering equipment setup and use, loading of DNA samples as well as concepts of molecule separation, gel analysis, and common electrophoresis applications.
How to Use an Analytical Balance
How to Use an Analytical Balance
Learn how to use an analytical balance. In this video, we'll explore the steps and techniques required to set up, take measurements, and clean the balance.
  •  
  • 1 of 5
  • >
Videos
View All
Gel Electrophoresis
Gel Electrophoresis
This animation explores the fundamentals of gel electrophoresis by covering equipment setup and use, loading of DNA samples as well as concepts of molecule separation, gel analysis, and common electrophoresis applications.
How to Use an Analytical Balance
How to Use an Analytical Balance
Learn how to use an analytical balance. In this video, we'll explore the steps and techniques required to set up, take measurements, and clean the balance.
Use and Care of a Microscope
Use and Care of a Microscope
Learn how to use a microscope, see it in action, and then head to the lab to practice working hands-on. In this video, Clarence (our rotifer microscopy expert), describes the functions and procedures for using a microscope including: Stage controls Coarse and fine focus and more...
  •  
  • 1 of 5
  • >
Videos
View All
Measuring Volume - Beakers, Cylinders, Erlenmeyer Flasks, & Volumetric Flasks
Measuring Volume - Beakers, Cylinders, Erlenmeyer Flasks, & Volumetric Flasks
BioNetwork's Greg Smith demonstrates the typical pieces of glassware used for measuring volume in a life science laboratory.
How does a spectrophotometer work?
How does a spectrophotometer work?
This short animation demonstrates the inner workings of a spectrophotometer. Here's how a spectrophotometer works. A lamp provides the source of light. The beam of light strikes the diffraction grating, which works like a prism and separates the light into its component wavelengths. The grating is
Bioprocessing Part 3: Purification
Bioprocessing Part 3: Purification
This video is the third in a series of three videos depicting the major stages of industrial-scale fermentation: fermentation, separation/recovery, and purification. This program describes the process by which a biological product—in this case, a pure, concentrated solution of Green Fluorescent
  •  
  • 1 of 5
  • >
Activities
View All
EASE (Effective Authentic Science Event)
EASE (Effective Authentic Science Event)
Would you like to host hundreds of prospective students on your college campus for a fun day of science and mystery?  EASE (Effective Authentic Science Event) is a one-day outreach program for middle school students with the goal of showing them that science is fun, and that the local community
What's that Flavor?!
What's that Flavor?!
Ever want to solve the mystery of the lollipop covered in question marks? Students will use spectrophotometers to analyze the amount of FD&C dyes in a sample mystery pop and compare it to other known flavors within the same bag. Using the powers of math and science, your class will shine a
Quantitative Spectrophotometry
Quantitative Spectrophotometry
Spectrophotometers measure the effect of a sample on a beam of light. This information can provide scientists with quantitative data regarding the amount of analyte present in a sample such as the amount of FD&C dyes found in popular foods and drinks. In this activity, students will create a
  •  
  • 1 of 2
  • >
Activities
View All
EASE (Effective Authentic Science Event)
EASE (Effective Authentic Science Event)
Would you like to host hundreds of prospective students on your college campus for a fun day of science and mystery?  EASE (Effective Authentic Science Event) is a one-day outreach program for middle school students with the goal of showing them that science is fun, and that the local community
What's that Flavor?!
What's that Flavor?!
Ever want to solve the mystery of the lollipop covered in question marks? Students will use spectrophotometers to analyze the amount of FD&C dyes in a sample mystery pop and compare it to other known flavors within the same bag. Using the powers of math and science, your class will shine a
Quantitative Spectrophotometry
Quantitative Spectrophotometry
Spectrophotometers measure the effect of a sample on a beam of light. This information can provide scientists with quantitative data regarding the amount of analyte present in a sample such as the amount of FD&C dyes found in popular foods and drinks. In this activity, students will create a
  •  
  • 1 of 2
  • >
Activities
View All
Super Sleuth Sampler
Super Sleuth Sampler
Your students will become super sleuths as they analyze different pieces of evidence to solve a crime. Beginner sleuths will progress through a series of stations to solve the mystery, while more advanced sleuths will concentrate their efforts on more sophisticated processes to determine the
EASE (Effective Authentic Science Event)
EASE (Effective Authentic Science Event)
Would you like to host hundreds of prospective students on your college campus for a fun day of science and mystery?  EASE (Effective Authentic Science Event) is a one-day outreach program for middle school students with the goal of showing them that science is fun, and that the local community
Quantitative Spectrophotometry
Quantitative Spectrophotometry
Spectrophotometers measure the effect of a sample on a beam of light. This information can provide scientists with quantitative data regarding the amount of analyte present in a sample such as the amount of FD&C dyes found in popular foods and drinks. In this activity, students will create a
  •  
  • 1 of 2
  • >