Chromatography in Theory & Practice

Chromatography in Theory & Practice

Chromatography is a critical operational step in many pharmaceutical downstream processes. In this course, you will learn chromatography fundamentals, design, operations, key mechanisms, and performance testing. Laboratory exercises will provide hands-on experience in both Ion-exchange and hydrophobic interactions chromatography including practice with equipment setup, column conditioning, performance testing and column operation. In addition, use of automation software and column packing will be demonstrated.

This course must be taught at a BioNetwork location.
Level: Beginner
Lecture Hours: 16.00
Lab Hours: 16.00
Total Contact Hours: 32.00

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Topics Include

This 4-day, 8-hour course covers the following  topics:

  • Protein Function
  • Chemistry of Amino Acids
  • Green Fluorescent Protein
  • Column Chromatography in Protein Purification
  • High Resolution Techniques
  • Adsorption Chromatography
  • Ion-exchange Chromatography
  • Hydrophobic Interaction Chromatography
  • Affinity Chromatography
  • Operational consideration
  • Scale up
  • Bubble traps
  • Types of Column Packing
  • Aktaprime Unit Operations
  • Performance, Troubleshooting, Maintenance
  • Cost of Chromatography
  • Chromatography Control Systems

Learning Objectives

  • Explain the science behind protein purification and typical operations and terminology related to downstream biopharmaceutical processing.
  • Identify the components of a small scale chromatography system and operate the system using two separation mechanisms.
  • Discuss the requirements for the stationary phase including resin chemistry.
  • Demonstrate proficiency with performance testing of column, troubleshooting and maintenance  of the chromatography system.

Audience

This course is designed for entry level bioprocess operators/technicians, incumbent workers as well as professionals in the biotechnology industry with experience in protein purification and characterization.

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