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Chromatography is a critical operational step in many pharmaceutical downstream processes. In this course, you will learn chromatography fundamentals, design, operations, key mechanisms, and performance testing. Laboratory exercises will provide hands-on experience in both Ion-exchange and hydrophobic interactions chromatography including practice with equipment setup, column conditioning, performance testing and column operation. In addition, use of automation software and column packing will be demonstrated.
This 4-day, 8-hour course covers the following topics:
- Protein Function
- Chemistry of Amino Acids
- Green Fluorescent Protein
- Column Chromatography in Protein Purification
- High Resolution Techniques
- Adsorption Chromatography
- Ion-exchange Chromatography
- Hydrophobic Interaction Chromatography
- Affinity Chromatography
- Operational consideration
- Scale up
- Bubble traps
- Types of Column Packing
- Aktaprime Unit Operations
- Performance, Troubleshooting, Maintenance
- Cost of Chromatography
- Chromatography Control Systems
- Explain the science behind protein purification and typical operations and terminology related to downstream biopharmaceutical processing.
- Identify the components of a small scale chromatography system and operate the system using two separation mechanisms.
- Discuss the requirements for the stationary phase including resin chemistry.
- Demonstrate proficiency with performance testing of column, troubleshooting and maintenance of the chromatography system.
This course is designed for entry level bioprocess operators/technicians, incumbent workers as well as professionals in the biotechnology industry with experience in protein purification and characterization.