Chromatography in Theory & Practice

This course must be taught at a BioNetwork location.

Request this Course

Request this Course

Submit this form to request this course to be scheduled.

14 + 0 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Chromatography in Theory & Practice
Level: Beginner
Lecture Hours: 16.00
Lab Hours: 16.00
Total Contact Hours: 32.00
Currently Offered
Chromatography in Theory & Practice - Class begins November 3, 2018

Description

Chromatography is a critical operational step in many pharmaceutical downstream processes. In this course, you will learn chromatography fundamentals, design, operations, key mechanisms, and performance testing. Laboratory exercises will provide hands-on experience in both Ion-exchange and hydrophobic interactions chromatography including practice with equipment setup, column conditioning, performance testing and column operation. In addition, use of automation software and column packing will be demonstrated.

Topics Include

This 4-day, 8-hour course covers the following  topics:

  • Protein Function
  • Chemistry of Amino Acids
  • Green Fluorescent Protein
  • Column Chromatography in Protein Purification
  • High Resolution Techniques
  • Adsorption Chromatography
  • Ion-exchange Chromatography
  • Hydrophobic Interaction Chromatography
  • Affinity Chromatography
  • Operational consideration
  • Scale up
  • Bubble traps
  • Types of Column Packing
  • Aktaprime Unit Operations
  • Performance, Troubleshooting, Maintenance
  • Cost of Chromatography
  • Chromatography Control Systems

Learning Objectives

  • Explain the science behind protein purification and typical operations and terminology related to downstream biopharmaceutical processing.
  • Identify the components of a small scale chromatography system and operate the system using two separation mechanisms.
  • Discuss the requirements for the stationary phase including resin chemistry.
  • Demonstrate proficiency with performance testing of column, troubleshooting and maintenance  of the chromatography system.

Audience

This course is designed for entry level bioprocess operators/technicians, incumbent workers as well as professionals in the biotechnology industry with experience in protein purification and characterization.

Current Courses
View All
September
27
HPLC in Theory & Practice
High performance liquid chromatography (HPLC) is a chemistry technique used to separate compounds of interest from a liquid mixture based on chemical and/or physical properties. When HPLC is coupled with a spectrophotometric detector, it can be used to quantify analytes to provide detailed
November
10
Sample Preparation for HPLC Analysis
Gain hands-on experience in sample preparation and purification techniques. You will learn to analyze biological samples for trace levels of drug and pesticide residues using HPLC (High Performance Liquid Chromatography). Students perform standard preparation, reagent preparation, sample
December
1
HPLC in Theory & Practice
High performance liquid chromatography (HPLC) is a chemistry technique used to separate compounds of interest from a liquid mixture based on chemical and/or physical properties. When HPLC is coupled with a spectrophotometric detector, it can be used to quantify analytes to provide detailed
Current Courses
View All
September
27
HPLC in Theory & Practice
High performance liquid chromatography (HPLC) is a chemistry technique used to separate compounds of interest from a liquid mixture based on chemical and/or physical properties. When HPLC is coupled with a spectrophotometric detector, it can be used to quantify analytes to provide detailed
November
10
Sample Preparation for HPLC Analysis
Gain hands-on experience in sample preparation and purification techniques. You will learn to analyze biological samples for trace levels of drug and pesticide residues using HPLC (High Performance Liquid Chromatography). Students perform standard preparation, reagent preparation, sample
December
1
HPLC in Theory & Practice
High performance liquid chromatography (HPLC) is a chemistry technique used to separate compounds of interest from a liquid mixture based on chemical and/or physical properties. When HPLC is coupled with a spectrophotometric detector, it can be used to quantify analytes to provide detailed
eLearning
View All
Green Fluorescent Protein (GFP)
Green Fluorescent Protein (GFP)
In this program, we’ll look at the production of Green Fluorescent Protein. GFP is used as a biological marker, and when attached to a drug it can provide researchers with a visual story of where the drug goes. It's a fluorescent dye that's very well tolerated by most cells and doesn't interfere
Good Laboratory Practices (GLP)
Good Laboratory Practices (GLP)
You’re the newest member of the Quality Assurance department at CATT - the Center for the Advancement of Toast Technology. Your mission is to audit the company and determine if it’s SOPs, notebooks, equipment, personnel, reagent labels, and test animals are GLP compliant.
High Performance Liquid Chromotography (HPLC)
High Performance Liquid Chromotography (HPLC)
Learn the high level concepts of High Performance Liquid Chromatography by finding out which has more caffeine - an energy drink or coffee? Learn how HPLC works by testing each drink, and then examine the resulting to chromatograms to find out.
  •  
  • 1 of 2
  • >
eLearning
View All
Green Fluorescent Protein (GFP)
Green Fluorescent Protein (GFP)
In this program, we’ll look at the production of Green Fluorescent Protein. GFP is used as a biological marker, and when attached to a drug it can provide researchers with a visual story of where the drug goes. It's a fluorescent dye that's very well tolerated by most cells and doesn't interfere
Good Laboratory Practices (GLP)
Good Laboratory Practices (GLP)
You’re the newest member of the Quality Assurance department at CATT - the Center for the Advancement of Toast Technology. Your mission is to audit the company and determine if it’s SOPs, notebooks, equipment, personnel, reagent labels, and test animals are GLP compliant.
High Performance Liquid Chromotography (HPLC)
High Performance Liquid Chromotography (HPLC)
Learn the high level concepts of High Performance Liquid Chromatography by finding out which has more caffeine - an energy drink or coffee? Learn how HPLC works by testing each drink, and then examine the resulting to chromatograms to find out.
  •  
  • 1 of 2
  • >
eLearning
View All
Good Laboratory Practices (GLP)
Good Laboratory Practices (GLP)
You’re the newest member of the Quality Assurance department at CATT - the Center for the Advancement of Toast Technology. Your mission is to audit the company and determine if it’s SOPs, notebooks, equipment, personnel, reagent labels, and test animals are GLP compliant.
High Performance Liquid Chromotography (HPLC)
High Performance Liquid Chromotography (HPLC)
Learn the high level concepts of High Performance Liquid Chromatography by finding out which has more caffeine - an energy drink or coffee? Learn how HPLC works by testing each drink, and then examine the resulting to chromatograms to find out.
Chromatography
Chromatography
This module includes, basics of Chromatography, affinity, ion exchange, size exclusion, part and functions associated with chromatography.
  •  
  • 1 of 2
  • >
Videos
View All
Spirit Tracker
Spirit Tracker
During filming of a new "Spirit Tracker" episode, the show's camera operator, Chelsea, goes missing.  The location is said to be haunted.  Is her disappearance the result of otherworldly forces, or is it foul play?  Play this video first, then have your students test the evidence provided in the
Bioprocessing Part 3: Purification
Bioprocessing Part 3: Purification
This video is the third in a series of three videos depicting the major stages of industrial-scale fermentation: fermentation, separation/recovery, and purification. This program describes the process by which a biological product—in this case, a pure, concentrated solution of Green Fluorescent
How does a spectrophotometer work?
How does a spectrophotometer work?
This short animation demonstrates the inner workings of a spectrophotometer. Here's how a spectrophotometer works. A lamp provides the source of light. The beam of light strikes the diffraction grating, which works like a prism and separates the light into its component wavelengths. The grating is
  •  
  • 1 of 2
  • >
Videos
View All
Spirit Tracker
Spirit Tracker
During filming of a new "Spirit Tracker" episode, the show's camera operator, Chelsea, goes missing.  The location is said to be haunted.  Is her disappearance the result of otherworldly forces, or is it foul play?  Play this video first, then have your students test the evidence provided in the
Bioprocessing Part 3: Purification
Bioprocessing Part 3: Purification
This video is the third in a series of three videos depicting the major stages of industrial-scale fermentation: fermentation, separation/recovery, and purification. This program describes the process by which a biological product—in this case, a pure, concentrated solution of Green Fluorescent
How does a spectrophotometer work?
How does a spectrophotometer work?
This short animation demonstrates the inner workings of a spectrophotometer. Here's how a spectrophotometer works. A lamp provides the source of light. The beam of light strikes the diffraction grating, which works like a prism and separates the light into its component wavelengths. The grating is
  •  
  • 1 of 2
  • >
Videos
View All
How does a spectrophotometer work?
How does a spectrophotometer work?
This short animation demonstrates the inner workings of a spectrophotometer. Here's how a spectrophotometer works. A lamp provides the source of light. The beam of light strikes the diffraction grating, which works like a prism and separates the light into its component wavelengths. The grating is
Bioprocessing Part 3: Purification
Bioprocessing Part 3: Purification
This video is the third in a series of three videos depicting the major stages of industrial-scale fermentation: fermentation, separation/recovery, and purification. This program describes the process by which a biological product—in this case, a pure, concentrated solution of Green Fluorescent
Bioprocessing: Join Us On The Edge
Bioprocessing: Join Us On The Edge
"Bioprocessing: Join Us On The Edge" is designed to educate beginning biotechnology students about specific career opportunities in industrial biotechnology by helping students to: * identify a career path * improve motivation to perform at a high level in class and laboratory settings * know
  •  
  • 1 of 2
  • >
Activities
View All
EASE (Effective Authentic Science Event)
EASE (Effective Authentic Science Event)
Would you like to host hundreds of prospective students on your college campus for a fun day of science and mystery?  EASE (Effective Authentic Science Event) is a one-day outreach program for middle school students with the goal of showing them that science is fun, and that the local community
Super Sleuth Sampler
Super Sleuth Sampler
Your students will become super sleuths as they analyze different pieces of evidence to solve a crime. Beginner sleuths will progress through a series of stations to solve the mystery, while more advanced sleuths will concentrate their efforts on more sophisticated processes to determine the
Activities
View All
EASE (Effective Authentic Science Event)
EASE (Effective Authentic Science Event)
Would you like to host hundreds of prospective students on your college campus for a fun day of science and mystery?  EASE (Effective Authentic Science Event) is a one-day outreach program for middle school students with the goal of showing them that science is fun, and that the local community
Super Sleuth Sampler
Super Sleuth Sampler
Your students will become super sleuths as they analyze different pieces of evidence to solve a crime. Beginner sleuths will progress through a series of stations to solve the mystery, while more advanced sleuths will concentrate their efforts on more sophisticated processes to determine the
Activities
View All
Super Sleuth Sampler
Super Sleuth Sampler
Your students will become super sleuths as they analyze different pieces of evidence to solve a crime. Beginner sleuths will progress through a series of stations to solve the mystery, while more advanced sleuths will concentrate their efforts on more sophisticated processes to determine the
EASE (Effective Authentic Science Event)
EASE (Effective Authentic Science Event)
Would you like to host hundreds of prospective students on your college campus for a fun day of science and mystery?  EASE (Effective Authentic Science Event) is a one-day outreach program for middle school students with the goal of showing them that science is fun, and that the local community