Advanced Microbiological Methods

This course must be taught at a BioNetwork location.

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Advanced Microbiological Methods
Level: Advanced
Lecture Hours: 10.00
Lab Hours: 10.00
Total Contact Hours: 20.00


Do you work in product manufacturing for pharmaceuticals, food, or dietary supplements? Are you looking to apply advanced microbiological identification techniques to your industry’s manufacturing processes as regulated by the FDA, USDA, and other agencies?

Consider this course.

Through informational lectures and practical laboratory exercises, you’ll cover theories and techniques of rapid bacteria and fungi detection, isolation, enumeration, and identification. Join in our discussions about molecular biology theory, biomolecules, and automated instrumentation alongside conventional microbiological methodology.

You’ll also talk about regulatory expectations, methods and rationale for environmental monitoring assessments, reporting, and microbiological quality control in the context of advanced instrumentation.

Topics Include

The course is 20 hours, conducted in two 8-hour sessions and a 4-hour session on third day and covers:

  • Introduction to Rapid Methodology/Instrumentation
  • Gold Standard Methods
  • Automated Enumeration Methods
    • Environmental monitoring
    • Statistical process controls (SPC)/    Quality control
  • Phenotypic Methods for Semi-Automated Microbial Detection/Identification
    • Automated
    • Gram stain
    • Biochemical-based ID
    • Sandwich ELISA detection
  • Advanced Microscopy Basics
    • Fluorescent gram stain
  • Automated Endotoxin Testing
  • Genotypic Methods for Semi-automated Microbial Isolation, Detection, and Identification
    • Polymerase chain reaction (PCR) sequencing
    • Best practices when performing molecular biology
    • Genetic material manipulation and loading instrumentation
  • Limitations of Rapid Instrumentation/Limits of Detection (LOD)
  • Microbiology Special Topics
    • Fungi, mycoplasma, and viruses
    • Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9
    • Resources


Learning Objectives

  • Define a wide range of advanced microbiological and molecular biology concepts and theories critical to the rapidly identifying microorganisms commonly found in an industrial setting.
  • Describe techniques used in automated biochemical identification, automated microbial enumeration and automated endotoxin detection.
  • Explain and demonstrate advanced techniques of isolation, detection, enumeration, and identification of bacteria and fungi commonly found in an industrial setting.
  • Summarize the Central Dogma of Biology, phenotypic versus genotypic methodologies, regulatory expectations, standard methodology, and resources available for interpreting advanced/rapid laboratory results.


We designed this course for experienced professionals, including:

  • Microbiologist
  • Microbiology Technician
  • Quality Assurance Laboratory Technician
  • Quality Control Technician
  • Environmental Monitoring Technician