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Students will learn how to monitor and control the experimental parameters of HPLC and GC and how to do calculations that permit the objective evaluation of a chromatogram. The course covers chromatographic theory including chromatographic parameters such as plate count, resolution, peak asymmetry and how they affect resolution. Also discussed is reversed phased chromatography including the nature of bonded phases, mixed mode effects, pH effects and developing separations. Students will also learn about gradient elution chromatography including instrument considerations, gradient benefits and problems, and gradient separation development.