HPLC Method Development Practices

HPLC Method Development Practices

Students will learn how to monitor and control the experimental parameters of HPLC and GC and how to do calculations that permit the objective evaluation of a chromatogram. The course covers chromatographic theory including chromatographic parameters such as plate count, resolution, peak asymmetry and how they affect resolution. Also discussed is reversed phased chromatography including the nature of bonded phases, mixed mode effects, pH effects and developing separations. Students will also learn about gradient elution chromatography including instrument considerations, gradient benefits and problems, and gradient separation development.

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Send us your interest if this course is currently not offered or the current schedule does not accomodate your schedule. It is often necessary to understand interest a semester in advance for planning and scheduling. Companies and organizations can often request a course if the group is large enough. This doesn't mean that we can make accommodations in every case. However, we will utilize and maximize our available resources as best as we can. Thanks for letting us know your interests and we will respond as soon as we can.

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