Fundamentals of High Performance Liquid Chromatography

Fundamentals of High Performance Liquid Chromatography

This course will teach students the fundamentals of High Performance Liquid Chromatography. Topics covered include an introduction to HPLC, overview and history, principles and theory, important terms and definitions, interpretation of chromatograms, qualitative and quantitative analysis, components of modern HPLC systems, mobile phase, pumps and injectors, HPLC columns and detectors, data recording systems, separation techniques, normal phase versus reversed-phase HPLC, isocratic versus gradient elution, common solvents used for HPLC mobile phases, column selection, sample preparation, HPLC method validation, applications of HPLC, other types of separation, and maintenance and troubleshooting. Successful completion of this course will prepare the student to understand and utilize HPLC applications in a life sciences laboratory.

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