Subculture of a Cell Line


As cultured cells grow and divide, they use up nutrients and eventually, cells will multiply to a point that they run out of space in the culture vessel.

Subculturing is the process of diluting and transferring the cells into more vessels with fresh culture medium so we can produce a new culture and the cells can continue growing.

In this video, we demonstrate the steps for propagating a culture by transferring NIH3T3 cells from a previous culture to a new culture vessel.