Restriction Digestion and Analysis of Lambda DNA

Restriction Digestion and Analysis of Lambda DNA
Target Audience: College
NCCCS Core Courses: BIO-110, BIO-111, BIO-112, BIO-175, BIO-275, BIO-280
Equipment Used: Electrophoresis Apparatus, Thermometer, Water Bath, Micropipette, Centrifuge

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Electrophoretic techniques that distinguish DNA fragments by size are essential in forensics and in the mapping of restriction sites within genes. With the Restriction Digestion and Analysis of Lambda DNA Kit, students use three different restriction enzymes to digest genomic DNA from lambda bacteriophage. By visualizing the effects of three different enzymes on identical samples of double-stranded DNA, students learn that different restriction enzymes recognize and cut different DNA sequences.